Zakharova I
Zakharova
Irina O.
PhD
OrcID: 0000-0001-8857-7012
ResearcherID: T-1394-2018
Research Gate: Irina-Zakharova
Scopus ID: 7004586810
eLibrary ID: 92757
E-Mail: zakhar@iephb.ru
Studies on changes of gangliosides and the composition of their fatty acids in rat brain during adaptation to cold laid the foundation for the investigation of the mechanism of the their neuroprotective action in the models of excitotoxicity and oxidative stress. Later the range of neuroprotectors to be probed was supplemented with alpha-tocopherol and insulin. I pay attention to the exproration of their ability to modulate the activity of the most important signaling pathways (Akt, Erk ½, GSK-3beta, AMPK) upon individual application and in various combinations with each other. In addition to signaling pathways, I am interested in mitochondrial transformation processes. The major methods in my hand are Western blotting, immunoprecipitation and PCR . Cell cultures and Wistar rats are the most frequently used objects in my experiments.
Articles
Zakharova I, Bayunova L, Avrova N / The Regulatory Role of Non-Coding RNAs in Autophagy-Dependent Ischemia–Reperfusion Injury of the Brain / Curr Issues Mol Biol 2025 47(6) : 462
DOIPubMed
DOIPubMed
Derkach K, Didenko Eg, Sorokoumov V, Zakharova I, Shpakov A / Low-molecular-weight ligand of the thyroid-stimulating hormone receptor with the activity of a partial agonist and a negative allosteric modulator / Dokl Biochem Biophys 2025 520(1) : 53-57
DOIPubMed
DOIPubMed
Derkach K, Gureev M, Babushkina A, Mikhaylov V, Zakharova I, Bakhtyukov A, Sorokoumov V, Novikov A, Krasavin M, Shpakov A, Balova I / Dual PTP1B/TC-PTP Inhibitors: Biological Evaluation of 3-(Hydroxymethyl)cinnoline-4(1H)-Ones / Int J Mol Sci 2023 24(5) : 4498
DOIPubMed
DOIPubMed
Zakharova I, Bayunova L, Zorina I, Sokolova T, Shpakov A, Avrova N / Insulin and α-Tocopherol Enhance the Protective Effect of Each Other on Brain Cortical Neurons under Oxidative Stress Conditions and in Rat Two-Vessel Forebrain Ischemia/Reperfusion Injury / International Journal of Molecular Sciences 2021 22(21) : 11768
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Bakhtyukov A, Derkach K, Sorokoumov V, Stepochkina A, Romanova I, Morina I, Zakharova I, Bayunova L, Shpakov A / The Effects of Separate and Combined Treatment of Male Rats with Type 2 Diabetes with Metformin and Orthosteric and Allosteric Agonists of Luteinizing Hormone Receptor on Steroidogenesis and Spermatogenesis / International Journal of Molecular Sciences 2021 23(1) : 198
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Zakharova I, Sokolova T, Bayunova L, Zorina I, Rychkova M, Shpakov A, Avrova N / Protective Effect of Insulin on Rat Cortical Neurons in Oxidative Stress and Its Dependence on the Modulation of Akt, GSK-3beta, ERK1/2, and AMPK Activities / International Journal of Molecular Sciences 2019 Jul 29;20(15) : E3702
DOIPubMed
DOIPubMed
Derkach K, Zakharova I, Zorina I, Bakhtyukov A, Romanova I, Bayunova L, Shpakov A / The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic mechanisms to this effect / PLOS One 2019 14(3) : e0213779
DOIPubMed
DOIPubMed
Sukhov I, Lebedeva M, Zakharova I, Derkach K, Bayunova L, Zorina I, Avrova N, Shpakov A / Intranasal administration of insulin and gangliosides improves spatial memory in rats with neonatal type 2 diabetes mellitus / Bull. Exp. Biol. Med. 2019 168(3) : 317-320
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Zorina I, Galkina O, Bayunova L, Zakharova I / Effect of Insulin on Lipid Peroxidation and Glutathione Levels in a Two-Vessel Occlusion Model of Rat Forebrain Ischemia Followed by Reperfusion / J Evol Biochem Phys 2019 55 : 333-335
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Zakharova I, Sokolova T, Bayunova L, Zorina I, Rychkova M, Shpakov A, Avrova N / The Protective Effect of Insulin on Rat Cortical Neurons in Oxidative Stress and Its Dependence on the Modulation of Akt, GSK-3beta, ERK1/2, and AMPK Activities / International Journal of Molecular Sciences 2019 20(15) : E3702
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Zorina I, Fokina E, Zakharova I, Bayunova L, Shpakov A / Features of the changes in lipid peroxidation and activity of Na+/K+-ATPase in the brain of the aged rats in the conditions of two-vessel cerebral ischemia/reperfusion / Advances in Gerontology 2019 32(6) : 941-947
PubMed
PubMed
Zakharova I, Sokolova T, Zorina I, Bayunova L, Rychkova M, Avrova N / Protective Effect of Insulin on Rat Cortical Neurons in Oxidative Stress and Its Dependence on Modulation of Protein Kinase B (Akt) Activity / J Evol Biochem Phys 2018 54 : 192–204
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Bayunova L, Zorina I, Zakharova I, Avrova N / Insulin Increases Viability of Neurons in Rat Cerebral Cortex and Normalizes Bax/Bcl-2 Ratio under Conditions of Oxidative Stress / Bull Exp Biol Med. 2018 May;165(1) : 14-17
DOIPubMed
DOIPubMed
Zorina I, Bayunova L, Zakharova I, Avrova N / The Dependence of the Protective Effect of Insulin on Its Concentration and Modulation of ERK1/2 Activity under the Conditions of Oxidative Stress in Cortical Neurons / Neurochemical Journal 2018 12 : 111-116
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Zorina I, Zakharova I, Bayunova L, Avrova N / Insulin Administration Prevents Accumulation of Conjugated Dienes and Trienes and Inactivation of Na+, K+-ATPase in the Rat Cerebral Cortex during Two-Vessel Forebrain Ischemia and Reperfusion / J Evol Biochem Phys 2018 54 : 246-249
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Zakharova I, Sokolova T, Vlasova Y, Bayunova L, Rychkova M, Avrova N / α-Tocopherol at Nanomolar Concentration Protects Cortical Neurons against Oxidative Stress / International Journal of Molecular Sciences 2017 Jan 21;18(1) : E216
DOIPubMed
DOIPubMed
Derkach K, Zakharova I, Romanova I, Zorina I, Mikhrina A, Shpakov A / Metabolic parameters and functional state of hypothalamic signaling systems in Ay mice with genetic predisposition to obesity and the effect of metformin / Dokl. Biochem. Biophys. 2017 477(1) : 377–381
DOIPubMed
DOIPubMed
Vlasova Y, Zakharova I, Avrova N / The effect of alpha-tocopherol and H2O2 on the mitochondrial membrane potential and Bax/Bcl-xL ratio in PC12 cells / Neurochemical Journal 2016 10(4) : 318-322
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Zakharova I, Sokolova T, Akhmetshina A, Avrova N / Alpha-tocopherol prevents a dramatic oxidative stress-induced decline of the Bcl-2 concentration in cortical neurons / Neurochemical Journal 2016 10(3) : 226-231
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Zakharova I, Sokolova T, Avrova N / Prevention by alpha-tocopherol of protein kinase B (Akt) inactivation in neurons of rat brain cortex, induced by oxidative stress, contributes to its protective effect / Journal of Evolutionary Biochemistry and Physiology 2016 52 : 173–176
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Zakharova I, Sokolova T, Akhmetshina A, Avrova N / Role of Protein Kinase Akt Activation in Protective Effect of Ganglioside GM1 on PC12 Cells Exposed to H2O2 / Bull. Exp. Biol. Med. 2015 159(5) : 610-613
DOIPubMed
DOIPubMed
Zakharova I, Sokolova T, Akhmetshina A, Avrova N / Alpha-tocopherol prevents long-term activation of ERK1/2 in neurons of the brain cortex under conditions of oxidative stress / Neurochemical Journal 2015 9(4) : 319-322
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