Komarova M

Komarova M

Komarova

Margarita S.

PhD

ResearcherID:  AAN-2166-2020
Research Gate:  Margarita_Komarova2
Scopus ID:  56581675500
eLibrary ID:  1070307

Komarova Margarita
Saint-Petersburg, Russia
Date of birth: 04.05.1990

EDUCATION
2013-2017
PhD student, Laboratory of Biophysics of Synaptic Processes (Dr. Denis Tikhonov`s lab), Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint-Petersburg, Russia
2011-2013
Master student, Department of Molecular Biotechnology,
St.-Petersburg State Technological Institute (Technical University), Saint-Petersburg, Russia. General Master’s program “Molecular and cellular biotechnology”
2007-2011
Bachelor student, Department of Molecular Biotechnology,
St.-Petersburg State Technological Institute (Technical University), Saint-Petersburg, Russia. Degree earned: Bachelor
of engineering and technology

ADDITIONAL COURSES
12-14 December,
2016
1st school ADFLIM “Modern methods of fluorescent imaging”, Moscow, Russia
26-28 July, 2017
2nd school ADFLIM “Modern methods of fluorescent imaging”, Saint-Petersburg, Russia

RESEARCH EXPERIENCE
2016-present
Research Fellow, Laboratory of Biophysics of Synaptic Processes, Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint-Petersburg, Russia (Dr. Denis Tikhonov`s lab)
Project: Search for physiological roles and new ligands of Acid-sensing ion channels. Methods: lipofectamine transfection, patch-clamp method (CHO cell culture, whole cell configuration)

2013-2016
Junior Research Fellow, Laboratory of Biophysics of Synaptic Processes, Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint-Petersburg, Russia, (Dr. Denis Tikhonov`s lab). PhD project: “Comparison of ligands` action mechanisms of TRPV1 receptors and ASICs”

2011-2013
Master research project: “Molecular pharmacology of antidepressants: mechanism of fluoxetine action on AMPA receptors”. Laboratory of Biophysics of Synaptic Processes, Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint-Petersburg, Russia
(Dr.Denis Tikhonov`s lab)

2010-2011
Bachelor research project: “Isolation of perlecan from human placenta”, Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg, Russia
(Dr.Georgiy Pinaev`s Lab)

RESEARCH SKILLS
Protein analysis
Western blotting, Dot-blotting, SDS-PAGE, Gel Filtration Chromatography, Ion Exchange Chromatography, Bradford Protein Assay
Mammal cell cultures
Passaging, lipofectamine transfection in CHO cell line, freezing for long-term conservation and defrosting
Electrophysiology
Patch-clamp (isolated neurons, CHO cell line)
Animals
Decapitation, brain slices

COMPUTER SKILLS
Research-related
PatchMaster, Clampfit, Microcal Origin
General
Microsoft Office Word, Excel, Power Point
Programming languages
Python (beginner), R (beginner)

TEACHING EXPERIENCE
Sep 2018-May 2019
Supervision of 4th year bachelor student in bachelor project
Sep 2019-current
Supervision of two 1st year master students in master projects

LANGUAGES
Russian (native), English (fluent)

 

 

 

Articles

Nikolaev M, Komarova M, Tikhonova T, Korosteleva A, Porapjeva N, Tikhonov D / Modulation of Proton-Gated Channels by Antidepressants / ACS Chem Neurosci 2019 Mar 20; 10(3) : 1636-1648
DOI
Komarova M, Porapjeva N, Nikolaev M / Non-Competitive but pH-Dependent Action of SB-366791 on Proton-Induced Activation of TRPV1 Receptors / Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 2018 12 : 107–113
DOI
Nikolaev M, Dorofeeva N, Komarova M, Korolkova Yu, Andreev Ya, Mosharova I, Grishin E, Tikhonov D, Kozlov S / TRPV1 activation power can switch an action mode for its polypeptide ligands / PLOS One 2017 12(5) : e0177077
DOI
Barygin O, Komarova M, Tikhonova T, Korosteleva A, Nikolaev M, Magazanik L, Tikhonov D / Complex action of tyramine, tryptamine and histamine on native and recombinant ASICs / Channels (Austin) 2017 Nov 2; 11(6) : 648-659
DOI
Barygin O, Komarova M, Tikhonova T, Tikhonov D / Non-classical mechanism of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor channel block by fluoxetine / Eur J Neurosci 2015 Apr; 41(7) : 869-77
DOI